Doubled Haploid(DH) Technology
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Project at a Glance Contents on the CD ROM
  • A doubled haploid (DH) is a genotype formed when haploid cells undergo
    chromosome doubling.
  • Artificial production of doubled haploids is important in plant
  • For doubled haploids to become a valuable breeding technique, it is necessary that success of doubled haploid production is not genotype dependent, the protocol used is efficient in time and cost.
  • Benefits of this technology are ,Double Haploid technology creates true-breeding lines in a single step, Combined DH & molecular marker technologies result in increased rates of genetic gain, Facilitates multiple trait stacking/pyramiding, Increases efficiency & probability of successful product development , Reduces time to market.
  • Double haploids are a product of modern technology which uses microspores to achieve a uniform parent line (homozygous) from very early generations of breeding material. This technique eliminates the need for several generations of breeding crosses.
  • Double haploids are also used to efficiently develop molecular mapping populations and ensure traits are uniformly expressed in a plant. This one step process replaces
    previous methods which could take up to six generations of plant growth.
  • Doubled-haploid technology enhances “forward breeding” by allowing hybrids to be bred with new traits (GMO and non-GMO) without locking up the germplasm, and by developing stacks without negative side effects.
  • The doubled-haploid method is much faster and can produce a new, genetically stable inbred line in one year.
  • The doubled-haploid method greatly reduces the time required to develop new parent inbreds.
  • The relevance of DHs to plant breeding has increased markedly in recent years owing to the development of protocols for 25 species.
  • Doubled haploidy already plays an important role in hybrid cultivar production of vegetables, and the potential for ornamental production is being vigorously examined.
  • The other advantages include development of large number of homo-zygous lines, efficient genetic analysis and development of markers for useful traits in less time.
  • Tobacco, rapeseed, and barley are the most responsive species for doubled haploid production.


  • Doubled Haploids improve plant breeding efficiency.
  • Doubled Haploids in Hybrid Maize Breeding.
  • A case focusing on doubled haploid technology.
  • Haploid production technology in wheat and higher plants.
  • A doubled haploid laboratory for Kansas wheat breeding.
  • Doubled-Haploid Breeding technology.


  • Growth and yield of F1 Hybrid peaches developed from Doubled Haploids.
  • Doubled Haploids in Plant Breeding.
  • Production of Doubled Haploid Brassica Napus Lines at Vida.
  • Utilization of Doubled Haploid Lines in wheat breeding programs.
  • Pathways to doubled haploidy.
  • Making wheat Doubled Haploids.
  • Double Haploids-Improve
    Efficiency of maize breeding.


  • Future plans of the sunflower "Doubled Haploid" Project.
  • Canola Breeding Centre Innovation.
  • New Breeding Tools impact Canadian commercial farmer fields.
  • Double Haploid Trials.


  • Method for producing Double haploid plants by gynogenesis.
  • Generating doubled haploid maize plants.
  • Induced Chromosome doubling in plants.
  • Highly transformable elite doubled haploid line PH17AW.
  • Doubled Haploid Cells, embryos and plants.
  • Chromosome Doubling Method.
  • Reverse Progeny Mapping.
  • Method of seed breeding using high throughput nondestructive seed sampling.
  • Method of producing doubled haploid oil palms.

Company Profile

  • Company from Iceland.
  • Company from Mexico.
  • Company from New York.
  • Company from USA.


  • Consultancy from UK.
  • Consultancy from California.
  • Online Consultancy.

Market Demands

  • Future Trends in Corn Genetics & Biotechnology.



  • Hybrid Maize Breeding with doubled haploid lines.
  • Improved Efficiency of doubled haploid generation in hexaploid triticale.
  • Production of doubled haploids in oil palm.
  • Optimization of Cucumber Doubled Haploid Line Production using induced parthenogenic embryos.
  • Utilization of Doubled Haploids in Winter Oilseed Rape.
  • Efficiency of microspore culture for doubled haploid production in the breeding .
  • Results of using zea method for doubled haploid production in wheat breeding.
  • Doubled haploid plants following colchicines treatment.
  • Selection strategies in hybrid maize breeding with doubled haploids.


  • Doubled Haploid Technology for quickly developing inbred corn lines offered at ISU.
  • Development of Improved Spice Crops using Double Haploid Technology.
  • Doubled Haploids in Wheat Breeding.
  • Optimum Hybrid Maize Breeding Strategies Using Doubled Haploids.
  • Genetic Analysis of Biomass Heterosis in Rapeseed.


  • Doubled Haploids Speed Development of drought tolerant maize for Africa.
  • Corn Breeding put on fast track.
  • Using Doubled Haploids to accelerate wheat variety development.
  • New Breeding Tools Impact Canadian Farmer Fields.
  • Doubled Haploid Lab Beats Projections.
  • CIMMYT Training Course on Doubled Haploid (DH) Technology in Maize Breeding.


  • Extension of spring wheat breeding using doubled haploids technology.
  • Use of Doubled Haploids in Wheat Breeding.
  • How to use doubled haploids to improve winter wheat webinar.
  • Assessment of wheat X maize doubled haploid technology for genetic improvement of New Zealand wheat.




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