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  • A culture medium is any material prepared for the growth of bacteria in a laboratory. Microbes that grow and multiply in or on a culture medium are known as a culture. Agar is a common solidifying agent for a culture medium.
  • Agar is a gel at room temperature, remaining firm at temperature as high as 65C. Agar melts at approximately 85C, a different temperature from that at which it solidifies, 32-40C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agars may have different gel strengths or degrees of stiffness.
  • Agar is typically used in a final concentration of 1-2% for solidifying culture media. Smaller quantities (0.05-0.5%) are used in media for motility studies (0.5% w/v) and for growth of anaerobes (0.1%) and microaerophiles.
  • Specifications for bacteriological grade agar include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors and relative absence of metabolically useful minerals and compounds.
  • Blood agar plate (BAP) Contains mammalian blood (usually sheep or horse), typically at a concentration of 510%. BAP are an enriched, differential media used to isolate fastidious organisms and detect hemolytic activity. β-hemolytic activity will show complete lysis of red blood cells surrounding colony, while α-hemolysis will only partially lyse hemoglobin and will appear green. γ-hemolysis (or non-hemolytic) is the term referring to a lack of hemolytic activity.
  • Blood agar is used to support the growth of fastidious organisms and to determine the type of hemolysis (destruction of red blood cell walls) an organism produces.
  • CDC Anaerobic Blood Agar is an enriched medium for the growth and partial identification of obligate anaerobes. It also supports good growth of aerobic, facultative anaerobic and microaerophilic organisms found in clinical materials if incubated under the appropriate conditions.
  • Tryptose Agar is used for cultivating a wide variety of fastidious microorganisms, particularly for isolating Brucella. Tryptose Agar with 5% bovine serum, with or without antibiotics, remains a standard plating medium for the isolation of brucellae. For isolation of Brucella stains from contaminated milk, crystal violet (gentian violet) can be added to Tryptose Agar to suppress gram-positive organisms. Tryptose media can be supplemented with thiamine or citrate for the cultivation and maintenance of fastidious aerobic and facultative microorganisms.
  • Coagulase Mannitol Agar is used for the differentiation of Staphylococcus aureus from other species based on coagulase production and mannitol utilization. Coagulase Mannitol Agar aids in the differentiation of staphylococci by indicating the presence of coagulase and the utilization of mannitol. Coagulase production is dependant on the presence of mannitol, a protein factor in the brain heart infusion and blood serum (plasma). During utilization of the mannitol, the pH of the medium drops, causing the bromcresol purple indicator to change from purple to yellow and producing yellow zones around these colonies. An opaque area of coagulated plasma forms around the colonies of organisms that also produce coagulase.
General Information
  • Agar and gram Staining
  • Agar plate
  • Blood agar
  • Culture Media
  • Blood Agar Plates and Hemolysis

Company Profile

  • German Company
  • Company from USA
  • Company from Suffolk
  • US Company
  • Company from Spain
  • Company from California
  • Company from UK
  • Indian Company
  • Scientific Company Providing Quality Laboratory Products

Preparation & Process

  • All About Agar
  • Blood Agar Base
  • Blood Agar Base No. 2
  • Production of High Quality Blood Agar
  • Use of Sodium Polyanethol Sulfonate in the Preparation of 5% Sheep Blood Agar Plates
  • Conventional Diagnostic Methods
  • Hemolysis - Blood Agar
  • Culture Methods

Applications

  • Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae
  • Isolation and Identification of Thermophilic campylobacters from diarrhoeal Children in Baghdad
  • Abolition of Swarming of Proteus by p-Nitrophenyl Glycerin: Application to Blood Agar Media
  • A Mutant of Bacillus Subtilis with High-Producing Surfactin by Ion Beam Implantation
  • Blood Agar and Mycobacterium tuberculosis: the End of a Dogma

Technology

  • New and Emerging Technology
  • An Agar Gel Diffusion Method for the Identification of Mosquito Blood- Meals
  • Pyrosequencing Bacillus anthracis
  • Microbial Cell Culture Technique and Morphological Analysis Technique
  • The agglutinability of Blood and Agar Strains of Typhoid Bacilli
  • Cost-Effectiveness of Blood Agar for Isolation of Mycobacteria

Standards

  • Charcoal Agar Blood With Cephalexin
  • Guidelines for Assuring Quality of
    Medical Microbiological Culture Media
  • Inoculation of culture media
  • Quality control of culture media in a microbiology laboratory
  • Quality Control for Microbiological Culture Media
  • Blood and chocolate agar

Project & Experts

  • Bacteria Growing in Blood Agar Petri Dishes
  • Nile tilapia, oreochromis niloticus, blood agar and the culture of fish bacterial pathogens
  • Testing Hemolytic Growth and Nutrient Levels Found Within
  • The Effect of Antiseptics on the Survival Rate of Different Types of Bacteria
  • The Effect of Antibacterial and Non-Antibacterial Hand Soap on the Amount of Bacteria on the Hands
  • The Effect of Mouthwash on Alpha Streptococcus
  • The Effects of Antibiotics on Bacterial Growth
  • Consultant from USA
  • US Consultant from Alabama
  • Consultant from New York
  • US Scientists

 

Properties & Functions
  • In vitro Antibacterial Comparison of Dentifrices Claiming Antigingivitis and Gum-Healing Properties
  • Detection of Pneumococci in Respiratory Secretions: Clinical Evaluation of Gentamicin Blood Agar
  • A Comparision of Columbia Blood Agar With or Without Oxolinic Acid for the Isolation of  Streptococci from Throat Swabs
  • Isolation of Bacteria from Ear, Nose and Throat
  • Susceptibilities of Mycobacterium tuberculosis to Isoniazid and Rifampin on Blood Agar
  • As a Bacterial Culture Medium, Citrated Sheep Blood Agar Is a Practical Alternative to Citrated Human Blood Agar in Laboratories of Developing Countries

Products

  • Azide Blood Agar Base
  • Blood Agra Base, Improved
  • Blood Agra Base w/Low PH
  • Campy Blood Agar
  • CDC Anaerobic Blood Agar
  • Columbia Blood Agar
  • Blood Glucose Agar
  • Cystine Tellurite Blood Agar
  • Tryptose Blood Agar Base
  • Blood Agar
  • Anaerobe Neomycin 5% Sheep Blood Agar
  • Coagulase Mannitol Agar

Material Safety Data Sheet

  • Blood Agar Base No. 2
  • Columbia CNA Blood Agar
  • Tryptose Blood Agar Base
  • Blood Agar
  • Sheep Blood Agar
  • Blood Agar, Prepared Microbiological Media
  • Blood Agar Base

Patent

  • Cell Culture Medium
  • Instant Culture Media and method of Sterilizing same
  • Microbial culture medium containing agar and iota carrageenan
  • Microscope slide having culture media and method for use
  • Novel Enrichments for Blood Culture Media
  • Peptides for use in Culture Media

Report & Market

  • Comparison of Mannitol Salt Agar and Blood Agar Plates for Identification and Susceptibility Testing of Staphylococcus aureus in Specimens from Cystic Fibrosis Patients
  • Evaluation of Crystal Violet Blood Agar for Primary Isolation and Identification of Group A β Haemolytic Streptococcus
  • A Study of Shelf-Life of Critical Culture Media
  • Culture Media and Supplements
  • Blood Agar, Chocolate Agar, and Mycobacterium tuberculosis
  • Isolation of Escherichia Coli from Raw Milk and Milk Products in Relation to Public Health Sold under Market Conditions at Tandojam
  • Hemolytic streptococci in Raw Market Milk
  • Rapid Methods and Automation in Microbiology: 25 years of
    Development and Predictions

Suppliers

  • Selling Leads of Culture Media
  • Suppliers of Microbiological Media & Sups
  • Global Bio Medical Suppliers
  • Blood Agar Plates suppliers
  • Manufacturers and Suppliers of Blood Agar Plates

 

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